M.Sc. IV Semester Microbiology (CBCS)
Paper III Microbial Biotechnology (Elective-I)
(4 HPW-4Credits)
Unit I
Fermentative production of industrial alcohol, uses, raw materials, microorganisms, inoculum
preparation, preparation of wort, fermentation and recovery.
Fermentative production of beer – Medium components, malt, malt adjuncts, hops, water.
Preparation of wort, mashing, wort boiling, microorganism, inoculum preparation, fermentation,
cold storage maturation, carbonation, packing and preservation.
Principles of wine making – Fruit selection, picking, crushing, sulphite addition, processing,
fermentation, aging and bottling.
Unit II
Fermentative production of citric acid, uses, microorganism, inoculum preparation, medium
preparation, fermentation, recovery and mechanism of citric acid production.
Fermentative production of vitamin B12 – Uses, structure of vit-B12, microorganisms, inoculum
preparation, medium preparation, fermentation and recovery.
Fermentative production of glutamic acid – Uses, microorganism, inoculum preparation,
production medium, fermentation and down stream processing.
Unit III
Antibiotics – Commercial production of benzyl penicillin, uses, microorganism, inoculum
preparation, production medium, fermentation, recovery and semi-synthetic penicillins.
Fermentative production of tetracyclines – uses, chlortetracycline, oxy-tetracycline, tetracycline
and semisynthetic tetracyclines, structures, microorganisms, inoculum preparation, production
medium, fermentation and recovery methods.
Unit IV
Production and application of microbial enzymes. – Amylases and proteases, uses,
microorganisms, inoculum preparation, production medium, fermentation and recovery
Steroid transformations – Substrates, typical structures, microorganisms, inoculum preparation,
11-hydroxylation, process and recovery.
Principles of vaccine production and types of vaccines
Microbial biopesticides
Microbial products from genetically modified (cloned) organisms eg. Insulin.
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