Cell and Molecular Biotechnology : notes

Unit I 

Cell cycle: Cell division regulation and cancer.

Role of protein Kinases in cell cycle. 

Programmedcell death.

Geno toxicity assays.

Signal transduction : G- Protein linked receptors. Concept of second messenger, cAMP & cGMP. Steroid/peptide hormone regulation, tissue specific regulation. Protein folding and the roles of Molecular chaperones. 

Unit II

Vectors in Molecular Biology, 

Artificial chromosomes, Enzymes, 

Polymerase chain reaction, 

DNA/Protein sequencing , 

rRNA/ Genomic/ c DNA Library construction and screening. 

Cloning Techniques: cloning in E-coli, 

Cloning in Bacillus subtilis, 

https://www.slideshare.net/AliaNajiha1/c4-mic210

Cloning in Yeast promoters, Vectors, 

cloning strategy, Transformation, Selection, Expression and detection of cloned genes.

simple Cloning Techniques:

Unit III

Molecular techniques: Analysis of Protein-protein and protein-DNA interactions.

Biochips (DNA chips and Protein chips).

Polymerase chain reaction and Quantitative real time PCR

DNA fingerprinting and DNA markers: RAPD, RFLP, A

FLP, Simple sequence repeat (SSR)

markers. Site directed mutagenesis,

 Reverse Genetics, Gene knock out – RNAi and Gene

silencing, 

Gene therapy.

Introduction to Omics techniques: Metagenomics, Transcriptomics and proteomics.